Then you take it for 2 months each year for the next 5 years. There is no proper research evidence that Tian Xian works as a treatment for cancer. We are concerned that these potential cures are often sold for a great deal of money. And people with cancer can be vulnerable. It is understandable that patients or relatives will try anything if they think it might work. We recommend that you talk to your doctor before buying or using any type of alternative therapy.
Find out what complementary therapies and alternative therapies are, why people with cancer might use them and whether they are safe.
Researching complementary and alternative therapies can be a challenge. But some high quality research is looking into complementary therapies to help people with cancer. About Cancer generously supported by Dangoor Education since You should always be aware of the possibly heavy metal contamination in most botanical medicines.
Once the herbal components are extracted from a polluted land or soils that are contaminated by heavy metals, they would bring harm to health despites its exquisite features. TXL products are strictly inspected by government departments, in terms of consistency, stability, safety, technology, GMP standard and etc. TXL has more than 26 years of experiences in serving more than , patients from 30 countries since TXL is able to nourish blood, strengthen immune system, improve digestive health, appetite and insomnia issue and etc.
For further enquiries, please contact our TXL Consultant. Mitochondrial membrane potential was assessed by 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'tetraethylbenzimidazolylcarbocyanine iodide JC-1 according to the manufacturer's protocol Biotium, USA.
A green filter was used for green-fluorescent monomer at depolarized membrane potentials and a red filter for orange-fluorescent J-aggregate at hyperpolarized membrane potentials. To measure the quantitative change of mitochondrial potential, we applied JC-1 with fluorescence plate reader.
Cells were washed twice with PBS to remove unbound dye. Six-week-old female nude mice were purchased from the Laboratory Animal Unit, University of Hong Kong and kept under sterile conditions in accordance with the institutional guidelines of animal care.
The body weights of all animals were recorded throughout the experiment to assess drug toxicity. Data were presented as mean and standard deviation SD. When one-way ANOVA showed significant differences among groups, Tukey's post hoc test was used to determine the specific pairs of groups that were statistically different.
Analysis was performed with the software SPSS version The results were consistent. TXL's inhibitory effects on cellular growth and apoptosis in HT cells. Apoptotic cell exhibiting characteristic chromatin condensation were observed by fluorescence microscopy. The experiment was repeated three times with similar results.
CTL: control. JC-1 staining was used to determine mitochondrial integrity. To quantify the change of mitochondrial potential, we applied JC-1 with fluorescence plate reader. The green to red fluorescence ratio significantly decreased at 48 hours in a dose-dependent manner Figure 2B.
TXL's effect on the depolarization of HT mitochondria. A JC-1 staining observed by fluorescence microscopy. TXL treated cells showed a majority of cells stained green dye due to low mitochondrial membrane potential. The cells were exposed to increasing concentrations of TXL. We studied the effects of TXL on the expression of Bax and Bcl-2 which are important for mitochondrial membrane permeablization. Cell lysates were prepared for western blot analysis.
TXL 0. Stability of mitochondrial membrane is influenced by the interactions among Bcl-2 family proteins, thereby affecting the release of cytochrome c from mitochondria to and subsequently accumulation in the cytosol [ 12 ].
The cytosol levels of cytochrome c in HT cells were examined with Western blot. Effect of TXL on cytochrome c. GAPDH antibody was used as control for equal loading. The relative expressions of proteins were quantified using Bio-Rad Quatity-One software. To confirm the induction of the mitochondrial-mediated apoptosis, we examined the activation of the intrinsic initiator caspase-9 and casapse-3 using western blot.
As shown in Figure 4 , caspases-9 in HT29 cells treated with TXL was activated, as judged by the decrease of the procaspases-9 and the increase of their cleavage products. TXL Induces caspase-3 and caspase-9 cleavage. To determine the antitumor efficacy of TXL as a single agent therapy, we examined the growth of HT cells in immunocompromised mice.
HT cells were injected in nude mice as described in Methods. In using this website you agree that neither Memorial Sloan Kettering nor any other party is or will be liable or otherwise responsible for any decision made or any action taken or any action not taken due to your use of any information presented at this website. This will help them manage your care and keep you safe. Tian Xian showed anticancer effects in laboratory studies and in animal models.
Clinical data are needed to confirm such effects. Ingredients may vary depending on manufacturers. Promoters of Tian Xian claim that it has immunomodulating and anticancer effects.
Laboratory studies show that it can increase the number of immune cells upon stimulation with antigens, and also has anticancer effects. A small study in breast cancer patients suggests that it may improve quality of life and alleviate side effects associated with chemotherapy. Larger controlled trials are needed to confirm these effects and to determine safety. Tian Xian is an extract of Chinese medicinal herbs used for cancer treatment in traditional medicine.
Ingredients may vary depending on manufacturers but usually include ginseng and astragalus as the main components.
Proponents claim that the anticancer effects of this extract are due to the immunomodulating properties of its constituents. In vitro and animal studies demonstrated that Tian Xian has immunomodulating 1 3 and anticancer 4 5 6 7 8 13 effects. In a small randomized controlled trial, Tian Xian liquid significantly improved QOL, decreased chemotherapy side effects, and demonstrated immunomodulating effects on lymphocytes in patients with refractory metastatic breast cancer.
Larger studies are needed to confirm safety and efficacy 9. No serious adverse effects have been reported from the use of Tian Xian liquid. However, patients should use caution because some of the herbs in Tian Xian may interact with prescription drugs due to cytochrome P 3A4 induction Future studies are needed to determine the extent to which this herbal remedy may either positively or negatively alter conventional anticancer therapy.
Tian Xian liquid increased proliferation of antigen-stimulated peripheral blood mononuclear cells and T cells isolated from patients with recurrent aphthous ulcers, likely via increased cytotoxic activity of NK cells, increased phagocytic activity of macrophages, anti-inflammatory effects, and immunopotentiation by the active constituents 1.
Suppression of gene expression and tumorigenicity of cancer stem-like hepatoma cells, as well as synergy with doxorubicin also occur through inhibition of multiple oncogenic signaling pathways 7.
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